Manual Klinische Chemie

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  1. Hauptnavigation
  2. Klinische Chemie
  3. Institut für Labormedizin – Stadtspital Triemli Zürich
  4. Die Schweizerische Gesellschaft für Klinische Chemie feiert 60 Jahre erfolgreiche Arbeit

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Erythrozyten Histamine plays a role in various cellular functions, including cell differentiation. The aim of this study was to determine the presence and to characterize the role of the endogenously produced histamine during in vitro dendritic cell DC differentiation induced by interleukin-4 IL-4 and granulocyte-monocyte colony stimulating factor GM-CSF.

The changes in intracellular histamine content, biosynthesis and gene expression of histidine decarboxylase were investigated during this process.

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Klinische Chemie

One also studied how histamine receptor antagonists and a histamine synthesis blocker influence the expression of differentiation antigens on the DC during in vitro maturation. During in vitro differentiation parallel culture incubations were performed by adding H1 receptor antagonist triprolidine, H2 receptor antagonist tiotidine, the tamoxifene derivate DPPE which blocks the intracellular binding of histamine, and an irreversible blocker of histidine decarboxylase, alpha-fluoromethyl histamine alpha-FMH.

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The results show simultaneous increase in both histidine decarboxylase level and histamine content during differentiation of elutriated monocytes toward DC. Both blockade of de novo histamine production by alpha-FMH and inhibition of histamine binding by H1 and H2 receptor antagonists, triprolidine and tiotidine, respectively markedly decreased CD40 expression and that of CD45 from the 3rd day of treatment.

Based on the data it is suggested that endogenous histamine is actively synthesized during cytokine-induced in vitro DC differentiation. Increase of histidine decarboxylase in human monocytes during macrophage colony stimulating factor induced in vitro phagocytic differentiation.

Possible role of histidine decarboxylase HDC and histamine in human in vitro monocyte-macrophage differentiation. Transport of lipids from Golgi to plasma membrane is defective in Tangier disease patients and Abc1-deficient mice. Gerhard Liebisch.

Institut für Labormedizin – Stadtspital Triemli Zürich

Leukocyte ABCA1 controls susceptibility to atherosclerosis and macrophage recruitment into tissues. ABCA1 functions ABCA1 functions as a facilitator of cellular cholesterol and phospholipid efflux, and its expression is induced during cholesterol uptake in macrophages. Our results provide evidence that leukocyte ABCA1 plays a critical role in the protection against atherosclerosis, and we identify ABCA1 as a leukocyte factor that controls the recruitment of inflammatory cells.

The gene encoding ATP-binding cassette transporter 1 is mutated in Tangier disease. Tangier disease TD is an autosomal recessive disorder of lipid metabolism.

Die Schweizerische Gesellschaft für Klinische Chemie feiert 60 Jahre erfolgreiche Arbeit

It is characterized by absence of plasma high-density lipoprotein HDL and deposition of cholesteryl esters in the reticulo-endothelial system with splenomegaly It is characterized by absence of plasma high-density lipoprotein HDL and deposition of cholesteryl esters in the reticulo-endothelial system with splenomegaly and enlargement of tonsils and lymph nodes. Although low HDL cholesterol is associated with an increased risk for coronary artery disease, this condition is not consistently found in TD pedigrees.

HDL-mediated cholesterol efflux and intracellular lipid trafficking and turnover are abnormal in TD fibroblasts, which have a reduced in vitro growth rate. The TD locus has been mapped to chromosome 9q Charalampos Aslanidis. An even larger complexity of myeloid cells with antigen. Cytofluorometric methods for assessing absolute numbers of cell subsets in blood.